Proper handling of TUD Gel & Clot Tube :

Step 1

• Proper blood draw (All our tubes are vacuum which could draw appropriate blood volume)

Step 2

• Proper inversion (Kindly follow our guideline for inversion of tube)

Inadequate mixing may result incomplete clotting and others technical fault.

Step 3

• Clotting period

CLOT for 30 minutes in a vertical position in a tube rack. Observe a dense clot.

• The recommended 30-minute minimum clotting time for the Gel & clot tube is based upon an intact clotting process.
• Insufficient clotting (short clotting time) can result in the formation of fibrin. This fibrin formation may interfere with barrier formation.
• Samples from certain populations of patients with impaired coagulation may require longer than 30 minutes to clot :
  

  - Blood from patients on anticoagulant therapy may require longer clotting time.
  - Blood from patients on high doses of heparin may not clot at all.
  - Certain diseases may require longer blood clotting times (e.g., liver disease).
  - Multiple Myeloma—the isolated myeloma globulin inhibits all three stages of fibrin formation: the proteolytic action of thrombin on fibrinogen, the aggregation of fibrin monomers, and the stabilization of fibrin by cross-linkages in the gamma and alpha chains

  
  It is recommended that serum be physically separated from contact with cells as soon as possible with a maximum time limit of 2 hours from the time of collection, unless conclusive evidence indicates that longer contact times do not contribute to error of the results.

Step 4

• Set the centrifuge speed and time to spin the tubes

Gel flow may be impeded if chilled before or during centrifugation. To optimize flow and prevent heating during centrifugation, set refrigerated centrifuges to 25°C (77°F)

Common Technical Fault

What is the caused of Fibrin Clot ?

• Improper inversion
• Without inversion immediately after blood draw
• Others

What is the caused of Hemolysis ?

• Shaking the tube contain with blood
• Press the needle from drawing the blood to the tube
• Re-centrifuge
• Centrifuge the blood tube which store in the refrigerator

Gel flow may be impeded if chilled before or during centrifugation. To optimize flow and prevent heating during centrifugation, set refrigerated centrifuges to 25°C (77°F)

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